In PCR,which of the following temperature ranges is used in the priming process?
A)90-95 °C
B)50-70 °C
C)60-80 °C
D)35-65 °C

Question

Quizplus · Accepted Answer

The temperature range used in the priming process is typically between 50-70 °C. This allows the primers to anneal, or bind, to the single-stranded DNA template. The higher temperature used in PCR (around 90-95 °C) is typically used for denaturing the DNA strands, while lower temperatures (around 60-80 °C) are used for extension and elongation of the new DNA strands. A temperature range of 35-65 °C is not typically used in the priming process of PCR.

In PCR,which of the Following Temperature Ranges Is Used in the Priming