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A Plasmid Vector and Chromosomal DNA Are Treated Separately with the Same

Question 6

Multiple Choice

A plasmid vector and chromosomal DNA are treated separately with the same restriction enzyme. Which of the following might occur if the digested plasmid and chromosomal DNA were incubated together?


A) The two sticky ends of the plasmid could hybridize back together, recircularizing.
B) The two sticky ends of the plasmid could hybridize to both sticky ends of the chromosomal DNA fragments.
C) The two sticky ends of the plasmid could hybridize back together and recircularize as well as
D) The two sticky ends of the plasmid could hybridize with two ends of another type of plasmid.
E) The two sticky ends of the vector would remain free or unhybridized.

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