During the tube gel electrophoresis phase of 2-D gel electrophoresis, proteins are separated based on their net charge at a given pH. Each protein will migrate to a point in the gel where its net charge is zero. This process is known as what?

Question

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Isoelectric focusing is the technique used in the first dimension of 2-D gel electrophoresis, where proteins are separated based on their isoelectric point (pI), the pH at which their net charge is zero.

During the Tube Gel Electrophoresis Phase of 2-D Gel Electrophoresis