High pressure liquid chromatography (HPLC) is not best for separating proteins because
A) it takes too long for high molecular weight molecules to elute
B) only highly acidic buffers can be used which alter proteins' primary structures
C) its required metal tubing interferes with protein purification
D) fast protein liquid chromatography (FPLC) runs at higher and more useful pressures
E) it cannot be accomplished in the cold
Correct Answer:
Verified
Q21: Dialysis is used to separate small molecules,
Q22: Dialysis tubing or bags
A) are made of
Q23: Ultrafiltration is a combination of
A) ion-exchange and
Q24: Chromatography
A) employs columns that are filled with
Q25: Which is NOT true of beads used
Q27: To monitor the protein concentration of samples
Q28: A protein rich in tryptophan would be
Q29: To facilitate quick analysis of eluents from
Q30: Analysis of proteins from the inner mitochondrial
Q31: Which is NOT important to size-exclusion chromatography?
A)
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