A researcher biotinylates a protein; this protein can then be purified by passing it through a column packed with resin coated with
A) avidin
B) divalent metal cations
C) DEAE (diethylaminoethyl groups)
D) phenyl groups
E) IgG
Correct Answer:
Verified
Q47: Ion exchange chromatography
I. separates proteins from a
Q48: Which is NOT true of a cellular
Q49: Carboxymethyl (CM) ion-exchange resin
A) bears a positive
Q50: A researcher adds a "tag" of several
Q51: Metal affinity chromatography (MAC) commonly uses divalent
Q52: Like ion exchange chromatography, MAC
A) eluent buffer
Q53: Biotin's cellular function includes
A) fatty acid biosynthesis
B)
Q54: Which is NOT true of biotin-avidin interaction?
A)
Q56: A biotinylated protein likely will not be
Q57: The most common immunoaffinity column packing is
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