EcoRI recognizes the sequence 5'-G$\downarrow$AATTC-3' (the arrow indicates the point of cleavage). Treatment of the following oligonucleotide with EcoRI would produce two oligonucleotides with sizes of _____ nucleotides containing _____ ends.
5'-AAGTCGATACAGAATTCGTACCTAG-3'

Question

Quizplus · Accepted Answer

EcoRI recognizes the sequence GAATTC and cuts between G and A, producing sticky ends. The given oligonucleotide contains one EcoRI site, dividing it into fragments of 12 and 13 nucleotides, both with sticky ends.

EcoRI Recognizes the Sequence 5'-G ↓\downarrow↓ AATTC-3' (The Arrow Indicates the Point of Cleavage)

EcoRI Recognizes the Sequence 5'-G $\downarrow$ AATTC-3' (The Arrow Indicates the Point of Cleavage)