Urea can be measured by incubation with urease followed by all of the following EXCEPT:
A) Formation of a colored product by reaction with diacetyl.
B) Measurement of increased conductivity.
C) Ion-selective electrode measurement of the ammonia produced.
D) NADH consumption in a reaction catalyzed by glutamate dehydrogenase.

Question

Quizplus · Accepted Answer

The correct methods for measuring urea after its conversion to ammonia by urease include measuring the increased conductivity due to the production of ions, using an ion-selective electrode to measure the ammonia directly, and quantifying NADH consumption in a reaction catalyzed by glutamate dehydrogenase, which is linked to the ammonia concentration. The formation of a colored product by reaction with diacetyl is not a standard method for measuring urea following urease treatment.

Urea Can Be Measured by Incubation with Urease Followed by All