If DNA is heated and then cooled,

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When DNA is heated, the hydrogen bonds between the base pairs break, leading to the separation of the two strands, a process known as denaturation. Upon cooling, these hydrogen bonds can reform if the conditions are suitable, allowing the strands to realign and reanneal, thus renaturing the DNA. This process does not inherently lead to new shapes, end-to-end reattachment, or the establishment of more chemical bonds than before heating.

If DNA Is Heated and Then Cooled