Polynucleotides have definite 5' and 3' ends (terminus).

Name: Polynucleotides have definite 5' and 3' ends (terminus).
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Polynucleotides, such as DNA and RNA, have a directionality defined by a 5' end, where the phosphate group is attached to the fifth carbon atom of the sugar ring, and a 3' end, where the hydroxyl group is attached to the third carbon atom of the sugar ring, giving them definite 5' and 3' ends.

Polynucleotides Have Definite 5' and 3' Ends (Terminus)