A characteristic that distinguishes between secondary and tertiary structures is the fact that hydrogen bonding in all secondary structures are between backbone -C=O and H-N- groups.

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Secondary structures, such as alpha-helices and beta-sheets, are characterized by hydrogen bonds between the backbone carbonyl oxygen (-C=O) and amide hydrogen (-H-N-) of amino acids, which is a key feature distinguishing them from tertiary structures that involve more complex folding and interactions, including side chain interactions.

A Characteristic That Distinguishes Between Secondary and Tertiary Structures Is