The saturation behaviour of enzyme systems (E + S) suggests that

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The saturation behavior of enzyme systems indicates that as substrate concentration increases, the rate of reaction eventually reaches a maximum where the enzyme is fully saturated with substrate. This suggests that different steps in the enzyme mechanism can become rate-determining based on the concentration of the substrate. Initially, the binding of the substrate to the enzyme might be rate-limiting, but as the substrate concentration increases and the enzyme becomes saturated, other steps such as catalysis or product release can become rate-limiting.

The Saturation Behaviour of Enzyme Systems (E + S) Suggests