A sample of cells with a total receptor concentration of 50 $\mu$ M was incubated with a ligand concentration of 100 $\mu$ M. Following the incubation period, the concentration of unbound receptors was determined to be 15 $\mu$ M. What is the K_d for the receptor-ligand interaction?

Question

Quizplus · Accepted Answer

The Kd can be calculated using the formula: Kd = [L][R]/[LR]. Here, [L] = 100 μM, [R] = 15 μM, and [LR] = 50 μM - 15 μM = 35 μM. Thus, Kd = (100 μM * 15 μM) / 35 μM = 42.86 μM, which is approximately 43 μM.

A Sample of Cells with a Total Receptor Concentration of 50