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When Is KM Considered to Be the Same as the Dissociation

Question 5

Multiple Choice

When is KM considered to be the same as the dissociation constant for the ES complex i.e., KM \approx [E] [S] / [ES].


A) ES \rarr E + P is fast compared to ES \rarr E + S
B) the turnover number is very large
C) kcat/KM is near the diffusion-controlled limit
D) k2 << k-1
E) KM can never be the same as the dissociation constant

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