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You Are Investigating a TRNA whose Charging Specificity Appears to Be

Question 53

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You are investigating a tRNA whose charging specificity appears to be affected by a U11:G24 to U11:A24 base pair in the D stem. Design an experiment using an in vitro reaction to show that changing this base-pair changes the charging specificity of the tRNA.

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Measure rates of GTP hydrolysis by mixin...

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