You have developed an in vitro assay for both splicing and polyadenylation. You generate in vitro radioactively labeled pre-mRNA substrates that either include a 5′ cap or lack the 5′ cap. You incubate these labeled pre-mRNA substrates with mammalian cell nuclear extract and run out the products on a sequencing gel. You then distinguish the products based on their relative size in the gel. You get the following results where the number of pluses is related to the relative amount of radioactivity found in that band on the gel:
Propose a hypothesis that explains these results.

Question

You have developed an in vitro assay for both splicing and polyadenylation. You generate in vitro radioactively labeled pre-mRNA substrates that either include a 5′ cap or lack the 5′ cap. You incubate these labeled pre-mRNA substrates with mammalian cell nuclear extract and run out the products on a sequencing gel. You then distinguish the products based on their relative size in the gel. You get the following results where the number of pluses is related to the relative amount of radioactivity found in that band on the gel:
  Propose a hypothesis that explains these results.

Quizplus · Accepted Answer

The Answer of You have developed an in vitro assay for both splicing and polyadenylation. You generate in vitro radioactively labeled pre-mRNA substrates that either include a 5′ cap or lack the 5′ cap. You incubate these labeled pre-mRNA substrates with mammalian cell nuclear extract and run out the products on a sequencing gel. You then distinguish the products based on their relative size in the gel. You get the following results where the number of pluses is related to the relative amount of radioactivity found in that band on the gel:
  Propose a hypothesis that explains these results.

You Have Developed an in Vitro Assay for Both Splicing