You have performed a bisulfite-PCR assay for distinguishing normal cytosine from 5-methyl cytosine. The starting sequence is: CGCGTAGCC. After sodium bisulfite treatment, PCR, and DNA sequencing, you obtain the following sequence: CGCGTAGTT. Which of the cytosines were methylated in the starting sequence? Explain your answer.

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The Answer of You have performed a bisulfite-PCR assay for distinguishing normal cytosine from 5-methyl cytosine. The starting sequence is: CGCGTAGCC. After sodium bisulfite treatment, PCR, and DNA sequencing, you obtain the following sequence: CGCGTAGTT. Which of the cytosines were methylated in the starting sequence? Explain your answer.

You Have Performed a Bisulfite-PCR Assay for Distinguishing Normal Cytosine