In an in vitro transcription assay, a G-less cassette could be used to do which of the following?

Question

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A G-less cassette in an in vitro transcription assay is designed to generate RNA transcripts of a defined length by excluding guanine (G) from the reaction, ensuring that transcription stops at the cassette due to the lack of GTP. It can also be used to pause RNA polymerase at a defined site in a template, as the absence of guanine nucleotides prevents further elongation of the RNA chain, effectively pausing the enzyme.

In an in Vitro Transcription Assay, a G-Less Cassette Could