You have identified a specific region in a virus that you suspect is bound by the host transcription factor Sp1. Which of the following methods could you use to test your hypothesis?

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All the listed methods can be used to test the hypothesis that a specific region in a virus is bound by the host transcription factor Sp1. Chromatin immunoprecipitation (ChIP) can be used to determine if Sp1 is bound to the viral DNA in vivo. Electromobility shift assay (EMSA) can be used to observe the binding of Sp1 to the viral DNA in vitro by the shift in mobility of DNA-protein complexes. DNase I footprinting can be used to identify the precise DNA sequence bound by Sp1 by protecting it from DNase I digestion.

You Have Identified a Specific Region in a Virus That