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(A) Outline the Steps That Would Be Necessary to Generate

Question 71

Essay

(a) Outline the steps that would be necessary to generate a cDNA library from all of the genes expressed by adenocarcinoma cells of a breast tumor. (b) How could tumor-specific transcripts be identified from this library? (Hint: think about how you could use non-cancerous breast epithelial cells as a comparison.)

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(a)203-204
(1) Isolate mRNAs from cells ...

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