The following is a physical map of a region you are mapping for RFLP analysis:
‪ The numbered vertical lines represent restriction sites recognized by SmaI. The red sites (2 and 3) are polymorphic, the others are not. You cut the DNA with SmaI, electrophorese the fragments, and Southern blot them to a membrane. You have a choice of two probes that recognize the DNA regions shown above: probe A, green line; probe B, blue line.
(a) Explain which probe you would use for analysis and why the other choice would be unsuitable.
(b) Give the sizes of bands you will detect in individuals homozygous for the following genotypes with respect to sites 2 and 3.

Question

The following is a physical map of a region you are mapping for RFLP analysis:
‪  The numbered vertical lines represent restriction sites recognized by SmaI. The red sites (2 and 3) are polymorphic, the others are not. You cut the DNA with SmaI, electrophorese the fragments, and Southern blot them to a membrane. You have a choice of two probes that recognize the DNA regions shown above: probe A, green line; probe B, blue line.
(a) Explain which probe you would use for analysis and why the other choice would be unsuitable.
(b) Give the sizes of bands you will detect in individuals homozygous for the following genotypes with respect to sites 2 and 3.

Quizplus · Accepted Answer

The Answer of The following is a physical map of a region you are mapping for RFLP analysis:
‪  The numbered vertical lines represent restriction sites recognized by SmaI. The red sites (2 and 3) are polymorphic, the others are not. You cut the DNA with SmaI, electrophorese the fragments, and Southern blot them to a membrane. You have a choice of two probes that recognize the DNA regions shown above: probe A, green line; probe B, blue line.
(a) Explain which probe you would use for analysis and why the other choice would be unsuitable.
(b) Give the sizes of bands you will detect in individuals homozygous for the following genotypes with respect to sites 2 and 3.

The Following Is a Physical Map of a Region You