A laboratory technique called "nick translation," in which nucleotides of a nicked dsDNA fragment are replaced with labeled nucleotides, takes advantage of which unique aspect of bacterial DNA Polymerase I?

Question

Quizplus · Accepted Answer

Nick translation utilizes the 5' to 3' exonuclease activity of DNA Polymerase I to remove nucleotides from the 5' end of a nick and replace them with labeled nucleotides using its 5' to 3' polymerization activity.

A Laboratory Technique Called "Nick Translation," in Which Nucleotides of a Nicked