Brief digestion of eukaryotic chromatin with micrococcal nuclease gives a visible ladder of bands on an agarose gel separated from each other by multiples of 180-200 bp. The fragment size represents

Question

Quizplus · Accepted Answer

The visible ladder of bands represents multiples of single nucleosomal repeat lengths of DNA, which includes DNA wrapped around the histone octamer and the linker DNA between nucleosomes. Each nucleosome typically consists of about 147 bp of DNA wrapped around histones and additional linker DNA, totaling approximately 180-200 bp per nucleosome.

Brief Digestion of Eukaryotic Chromatin with Micrococcal Nuclease Gives a Visible