The DNA duplexes below are denatured and then allowed to reanneal. Which of the two molecules would have the highest T_m? Which of the two is least likely to re-form the original structure? Why?
(a) 5′-ATATCATATGATATGTA-3′
3′-TATAGTATACTATACAT-5′
(b) 5′-CGGTACTCGTGCAGGT-3′
3′-GCCATGAGCACGTCCA-5′

Question

Quizplus · Accepted Answer

The Answer of The DNA duplexes below are denatured and then allowed to reanneal. Which of the two molecules would have the highest T_m? Which of the two is least likely to re-form the original structure? Why? (a) 5′-ATATCATATGATATGTA-3′ 3′-TATAGTATACTATACAT-5′ (b) 5′-CGGTACTCGTGCAGGT-3′ 3′-GCCATGAGCACGTCCA-5′

The DNA Duplexes Below Are Denatured and Then Allowed to Reanneal