A trait of plants that is of significant importance in plant biotechnology is the ability to regenerate a whole plant from a single plant cell. When growing undifferentiated tissue in the lab, how can such cells be maintained indefinitely?
A) All the options listed here are correct
B) Subculturing
C) By growing tissue on a synthetic growth medium that provides nutrients, carbohydrates and water
D) By growing tissue in an aseptic environment
E) By transferring tissue produced by wounding to a growth medium

Question

Quizplus · Accepted Answer

All the options listed (B, C, D, E) are correct methods to maintain undifferentiated plant tissue (also known as callus) indefinitely in the lab. Subculturing involves periodically transferring the tissue to fresh growth medium to provide new nutrients and space for growth. Using a synthetic growth medium ensures the cells receive all necessary nutrients, carbohydrates, and water. An aseptic environment prevents contamination by microorganisms. Transferring tissue produced by wounding to a growth medium can initiate and maintain callus formation.

A Trait of Plants That Is of Significant Importance in Plant