Complementary DNA (cDNA) libraries are made
A) because DNA in eukaryotes cannot be cloned.
B) to allow analysis of the coding gene regions.
C) as there are no restriction enzymes for non-coding regions of the genome.
D) by using restriction endonuclease digestion of DNA and then ligation of the fragments into the genome.
E) because they are easier to sequence than genomic DNA libraries.

Question

Quizplus · Accepted Answer

Complementary DNA (cDNA) libraries are created to focus on the coding regions of the genome, which are the parts of the genome that are transcribed into mRNA and then translated into proteins. This is achieved by reverse transcribing mRNA, thus ensuring that the library represents only the expressed genes of an organism at a specific time, under specific conditions.

Complementary DNA (CDNA) Libraries Are Made