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A Linear Piece of DNA Is Cut with Restriction Enzymes

Question 16

Multiple Choice

A linear piece of DNA is cut with restriction enzymes to give the following fragments of DNA:
HindIII 3.5 kb and 2 kb
EcoRI 1 kb and 4.5 kb
HindIII and EcoRI 1 kb 2.5 kb and 2 kb
Which of the following restriction maps shows the positions of the restriction enzyme cutting sites, and the sizes of the resulting fragments?


A) 1 kb 2.5 kb 2 kb
___|__________|________
EcoRI HindIII
B) 1 kb 2 kb 2.5 kb
___|________|__________
EcoRI HindIII
C) 1 kb 4.5 kb
___|__________________
HindIII
D) 1 kb 2.5 kb 4.5 kb
___|__________|__________________
HindIII EcoRI
E) 1 kb 3.5 kb 2 kb 4.5 kb
___|______|____|______________
EcoR1 HindIII EcoR1 HindIII

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