You Are Analysing Two Individual Genomes and Want to Determine

You are analysing two individual genomes and want to determine if there are any differences in their respective DNA sequences. You hypothesise that the sequences have only very minor single base pair changes. What method would you use to test this hypothesis and why?

A) Northern blots of restriction endonuclease digests, as single base pair changes would alter the recognition sites of the enzymes, thus producing different banding patterns
B) RFLP or Restriction Fragment Length Polymorphism analysis, as a lack of polymorphisms will indicate there are single base pair differences
C) Southern blots of restriction endonuclease digests, as single base pair changes would alter the recognition sites of the enzymes, thus producing different banding patterns
D) By probing with a radio-labelled probe that is complementary to the polymorphic sequences, as the probe will only bind to tandem repeats
E) Using microsatellite analysis via specific primers designed to anneal either side of the single base pair changes, as this would selectively amplify polymorphic sequences which could then be identified