How did Otto Loewi demonstrate that neurons communicate by releasing chemicals?

Question

How did Otto Loewi demonstrate that neurons communicate by releasing chemicals?​

Quizplus · Accepted Answer

Otto Loewi's experiment involved transferring fluid from one frog's heart to another to demonstrate that neurons communicate by releasing chemicals. He collected fluid from the first frog's heart and transferred it to an isolated second frog's heart, which caused a decrease in heart rate. This fluid was found to contain a neurotransmitter, which was later identified as acetylcholine, confirming the idea of chemical communication between neurons.

How Did Otto Loewi Demonstrate That Neurons Communicate by Releasing