Suppose the absorbance for a known solution was calculated to be 0.333.The solution was measured on a spectrophotometer, and the measured absorbance was 0.321.The solution was prepared again and gave the same absorbance reading of 0.321.Which of the following explanations would most accurately explain the difference between the calculated absorbance and the measured absorbance?

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Quizplus · Accepted Answer

The bandpass is a range of wavelengths that pass through the sample and are measured by the detector.If the molar absorptivity changes over the bandpass of the instrument, which is common, there is less absorbance observed than expected for the concentration of absorber present.This condition results in lower than expected absorbance because the observed absorbance is the integral of absorbance over the band of wavelengths actually passing through the cuvette.

Suppose the Absorbance for a Known Solution Was Calculated to Be