Considering the chromatographic separation of two solutes that are present in a mixture at the same concentration and produce the same detector response (i.e., have the same molar absorptivity), the solute that has a higher affinity for the stationary phase will have a ______ retention time and a ______ detector response than the solute that has a higher affinity for the mobile phase.

Question

Quizplus · Accepted Answer

Solutes that have a higher affinity for the chromatographic stationary phase will be retained by the system longer, resulting in a greater retention time.As this solute stays on the column longer, the solute will be subject to band spreading.As a result of band spreading, the peak height will decrease, resulting in a broader and shorter zone that has a lesser detector response.

Considering the Chromatographic Separation of Two Solutes That Are Present