It is known that pH influences the charge of an analyte and, if set appropriately, can enhance electrophoretic mobility.In an attempt to improve separation of serum proteins, the analyst set the pH of the buffer at an extremely basic level.Following electrophoresis, it was determined that the desired results were not achieved.Which of the following explains why the modification was not successful?

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The pH of the electrophoretic buffer should always be selected to give an optimum net charge for maximal separation.A very basic buffer will result in poorer electrophoretic separation, because there will be an increase in the fraction of current carried by the hydroxyl ions.Also, the very basic pH may have an effect on the structure of the proteins (either denaturing or dissociating them), resulting in undesirable electrophoretic results.

It Is Known That PH Influences the Charge of an Analyte