What will be the effect on the final electrophoretic pattern when horizontal electrophoresis is used and the surface of the medium is open to air?

Question

Quizplus · Accepted Answer

As evaporation occurs from the center of the medium, solvent will move from each buffer reservoir toward the center of the medium.Sample components that are migrating on the side of the medium to which the sample was applied will be subjected to buffer flow, moving them closer to the center of the medium.Sample components that are migrating beyond the center will encounter opposing buffer flow, causing these components to be slowed in their movement.Thus the migration of slow-moving components will be enhanced by the flow of buffer from one reservoir, and the migration of fast-moving components will be opposed by the flow of buffer from the other reservoir.The result is a compression of the electrophoretic separation.This is also known as wick flow.

What Will Be the Effect on the Final Electrophoretic Pattern