What is the most important variable to control during the measurement of ionized calcium by ion-selective electrode methodology?

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Ion-selective electrode technology is ideally suited for the measurement of ionized calcium.Ionized calcium is the physiologically active form of calcium, and an accurate determination of this level is extremely useful clinically.The most important variable associated with this measurement is the control of pH.As the pH changes, the percentage of ionized calcium will change.

What Is the Most Important Variable to Control During the Measurement