The mutation-detection technique that incorporates a set of immobilized DNA probes for different sequence variants to which PCR amplified DNA is added is known as:

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A simple way to type PCR-amplified DNA is the reverse dot blot technique.In this technique, a set of DNA probes for different sequence variants in the amplified region is immobilized in discrete dots on a membrane.The membrane is incubated with a solution of the amplified DNA, followed by a washing step.A positive match for one or more of the probes can be detected colorimetrically.Commercial kits are available that include prepared membranes dotted with a standard set of probes for the locus, along with dots for control probes.Reverse dot blot is the method most commonly used in forensic PCR applications.

The Mutation-Detection Technique That Incorporates a Set of Immobilized DNA