Various species of lightning bugs have their own characteristic patterns of light flashes. Females flash an answering code to the males' code, and mating follows the recognition. This is an example of a genetic isolating mechanism called

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Behavioral isolation is a type of prezygotic barrier where different species have distinct behaviors that prevent them from mating. In the case of lightning bugs, the unique patterns of light flashes between males and females of the same species serve as a mechanism to ensure mating occurs only within their species, thus preventing interbreeding with other species.

Various Species of Lightning Bugs Have Their Own Characteristic Patterns