You have identified a molecule, molecule X, that can bind to the ATP binding site of a specific protein kinase, and prevent ATP binding. You are conducting a kinase assay using this protein kinase and a known substrate of this kinase, and have set up the reaction using the proper conditions and co-factors to allow for optimal protein kinase activity. If you add molecule X to the reaction, what do you predict will be the outcome?

Question

Quizplus · Accepted Answer

Molecule X competes with ATP for binding to the ATP binding site of the kinase, preventing ATP from binding and thus reducing the phosphorylation of the substrate.

You Have Identified a Molecule, Molecule X, That Can Bind