Researchers pulsed rapidly dividing cultured cells for 30 minutes with radioactive thymidine.The cells were then exposed to a solution containing non-radiolabeled thymidine.Cells were analyzed at 2-hour intervals.At the 2-hour time point,no cells appeared to be dividing.Only after 4 hours did some labeled cells appear to be in M phase.This result can be explained in the following way:
A)Cultured cells all divide at the same time,and none synthesized DNA during the 30-minute labeling period.
B)The cells were arrested in a nondividing state because of the treatment and could not enter M phase until several hours after the label was removed.
C)Synthesis (S)phase is lengthy-about 12 hours in most cell types-and the radioactive thymidine was not present long enough for most cells to be labeled.
D)There seems to be a gap or a lag in the cell cycle,between the synthesis of DNA and cell division.

Question

Quizplus · Accepted Answer

The fact that there was a 2-hour delay between the end of the labeling period and the start of M phase suggests that there is a gap or lag in the cell cycle between DNA synthesis and cell division, which is known as the G2 phase. This delay is consistent with the time required for the cells to complete S phase and enter G2 phase before entering M phase.

Researchers Pulsed Rapidly Dividing Cultured Cells for 30 Minutes with Radioactive