What is the right progression of steps for a direct-sequencing experiment?
A)Lyse cells,purify DNA,amplify genes by PCR,and insert genes into plasmids.
B)Lyse cells,insert genes into plasmid,purify DNA,and amplify genes by PCR.
C)Lyse cells,insert genes into plasmid,amplify genes by PCR,and purify DNA.
D)Purify DNA,amplify genes by PCR,lyse cells,and insert genes into plasmid.

Question

Quizplus · Accepted Answer

In a direct-sequencing experiment, the typical progression is to first lyse cells to release the DNA, then purify the DNA, amplify the genes of interest using PCR, and finally, if needed, insert the amplified genes into plasmids for further analysis or manipulation.

What Is the Right Progression of Steps for a Direct-Sequencing