An Organic Solvent Widely Used in Industrial Processes 1,1,1-Trichloroethane (TCA)

An organic solvent widely used in industrial processes 1,1,1-trichloroethane (TCA) is a major environmental pollutant affecting human health and damaging the ozone layer.Recently researchers isolated the first bacteria able to degrade TCA.They found that the bacteria use hydrogen as an electron donor,TCA as electron acceptor,and acetate as a carbon source.Based on the preceding information,deduce the method used to isolate these bacteria.

A) Gram staining
B) enrichment culture
C) direct sequencing
D) seeding