You have been happily growing your bacterial cultures for many weeks.One day,you discover that one of your cultures has lysed.Which of the following experiments will indicate that a bacteriophage is responsible for the cell lysis?
A) You filter the lysed culture through a 0.3-micrometer filter and use the filtrate to infect more cells.After 45 minutes,the second bacterial culture lyses.
B) You examine the lysed culture using the electron microscope and discover you have many particles about 50-nanometer long,with a head,tail,and tail spikes.
C) You filter the lysed culture through a 0.3-micrometer filter and add fresh growth media.After 24 hours of incubation,the growth media is cloudy,like a bacterial culture.
D) A and B.
E) B and C.
Correct Answer:
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