You Are Carrying Out Mutational Studies on Membrane-Associated Proteins

You are carrying out mutational studies on membrane-associated proteins. You are particularly interested in proteins that pass through the plasma membrane of cells. Alteration of which of the following properties of an integral membrane protein would most likely interfere with its ability to become membrane associated?

A) the ability to bind carbohydrates on both sides of the membrane
B) the ability to move laterally in the plane of the membrane
C) the ability to translocate across the bilayer of the membrane when the temperature is increased
D) flip-flopping across the bilayer of the membrane when the temperature is increased
E) high solubility in strong basic solutions