Which of the following is correct regarding the Michaelis-Menten constant,Km,for this kinetic mechanism? A)K_m is numerically equal to the substrate concentration required to achieve one half the maximum velocity. B)Its defined as K_m = k₁/(k_-1 + k₂). C)K_m is approximately equal to the dissociation constant for the enzyme-substrate complex to E + P. D)The numeric value of Km has the units of mole^-1.

Question

Quizplus · Accepted Answer

The Michaelis-Menten constant,Km, is defined as the substrate concentration at which the reaction rate is half of its maximum value. Therefore, option A is correct. Option B is actually the equation for the Michaelis-Menten constant derived from the kinetic mechanism. Option C is incorrect because the dissociation constant for the enzyme-substrate complex is denoted as Kd and is not the same as Km. Option D is also incorrect because the units of Km are moles per liter (or molarity), and not mole^-1.

Which of the Following Is Correct Regarding the Michaelis-Menten Constant,Km,for