For an enzyme-catalyzed reaction,the initial velocity was determined at 2 different concentrations of the substrate.Which of the following would be closest to the value of Vmₐₓ?

A)0.32 mM/min
B)0.67 mM/min
C)1.5 mM/min
D)3.19 mM/min

Question

For an enzyme-catalyzed reaction,the initial velocity was determined at 2 different concentrations of the substrate.Which of the following would be closest to the value of Vmₐₓ?

A)0.32 mM/min 
B)0.67 mM/min 
C)1.5 mM/min 
D)3.19 mM/min

Quizplus · Accepted Answer

Vmₐₓ is the maximum velocity of the reaction and is achieved when all the enzyme active sites are saturated with substrate. To approximate Vmₐₓ, we need to look for the highest initial velocity observed in the experiment. From the given choices, the highest initial velocity is 3.19 mM/min (option D), which is closest to Vmₐₓ.

For an Enzyme-Catalyzed Reaction,the Initial Velocity Was Determined at 2