Which method(s) can be used to produce mice that lack brain serotonin throughout life, beginning with embryonic development?
A) Administering the tryptophan hydroxylase inhibitor PCPA
B) Knocking out the gene for tryptophan hydroxylase 2
C) Preventing the normal differentiation of cells that are destined to become serotonergic neurons
D) Both b and c

Question

Quizplus · Accepted Answer

Methods B and C directly target the genetic or developmental processes responsible for serotonin production. Knocking out the gene for tryptophan hydroxylase 2 (B) eliminates the enzyme necessary for serotonin synthesis, ensuring mice lack brain serotonin from development. Preventing the differentiation of serotonergic neurons (C) stops these cells from producing serotonin throughout the mouse's life.

Which Method(s) Can Be Used to Produce Mice That Lack