The hyperchromic shift occurs when dsDNA is denatured.At what wavelength is there an increase in the absorption as a result of this shift?
A)200 nm
B)220 nm
C)240 nm
D)260 nm

Question

Quizplus · Accepted Answer

The hyperchromic shift is the increase in the absorption of UV light at a wavelength of 260 nm due to the denaturation of dsDNA into ssDNA. Therefore, the best choice is D, 260 nm. The other wavelengths listed, 200 nm, 220 nm, and 240 nm, are not associated with the hyperchromic shift.

The Hyperchromic Shift Occurs When DsDNA Is Denatured