In order for transcription to begin,the DNA-duplex must be "opened" to allow RNA polymerase access to single-stranded template.Which of the following facilitates "opening" of DNA-duplex?
A)high melting temperature,Tm,in the Pribnow box
B)G:C-rich DNA duplex
C)binding of RNA polymerase s-subuni
D)positive supercoiling

Question

Quizplus · Accepted Answer

Binding of RNA polymerase s-subunit facilitates the opening of the DNA duplex, allowing access to the single-stranded template. The s-subunit recognizes the promoter region and initiates the transcription process. A high melting temperature (Tm) in the Pribnow box or a G:C-rich DNA duplex may affect the stability of the DNA duplex but does not directly facilitate its opening. Positive supercoiling can also facilitate the formation of the open complex for transcription, but it is not the only factor involved in the process.

In Order for Transcription to Begin,the DNA-Duplex Must Be "Opened