Which of the following techniques can be used to identify RBP-mRNA interactions?

Question

Quizplus · Accepted Answer

RNA-EMSA (electrophoretic mobility shift assay) is a technique that is commonly used to study RNA-binding protein (RBP)-mRNA interactions. It involves incubating labeled RNA with a purified RBP and running the mixture on a non-denaturing polyacrylamide gel. The gel is then visualized to detect changes in RNA mobility caused by RBP binding. SDS-PAGE, Southern blot analysis, and RT-PCR are not typically used to study RBP-mRNA interactions.

Which of the Following Techniques Can Be Used to Identify