Which of the Following Is Not True of Biotin

Which of the following is not true of biotin?

A) Biotin is a fat-soluble vitamin.
B) Biotin does not need to be included in a person's diet.
C) Biotin is required by enzymes that catalyze the carboxylation of an alpha-carbon.
D) Biotin-requiring enzymes also require bicarbonate, Mg²⁺ and ATP.
E) All biotin-requiring enzymes follow the same steps: activation of bicarbonate by ATP; reaction of biotin, with activated bicarbonate; and transfer of CO₂ to the substrate.