The enzyme EcoRI cuts between the G and the A in the sequence GAATTC.The enzyme BamHI cuts between adjacent G nucleotides in the sequence GGATCC.The sticky ends that are generated by the two enzymes are complementary and will stick together.

Question

Quizplus · Accepted Answer

EcoRI generates sticky ends with a 5' overhang of AATT, while BamHI generates sticky ends with a 5' overhang of GATC, which are not complementary to each other.

The Enzyme EcoRI Cuts Between the G and the a in the Sequence