Which of the following procedures would identify the enzyme that catalyzes the carboxylation of ribulose-1,5-bisphosphate?
A) irradiating a leaf extract with red light
B) introducing radiolabeled carbon dioxide into a plant extract and determining which molecules become radiolabeled
C) purifying a variety of proteins from plant extracts and testing each protein individually to see if it can carboxylate ribulose-1,5-bisphosphate
D) differential sedimentation of a protein extract

Question

Quizplus · Accepted Answer

Purifying a variety of proteins from plant extracts and testing each protein individually is the most appropriate method for identifying the specific enzyme responsible for the carboxylation of ribulose-1,5-bisphosphate. This is because the enzyme is likely to be present in low abundance in plant extracts, so isolating and testing each protein individually is necessary to determine which one is responsible for the reaction. The other methods listed are not specific enough to identify a single enzyme, as they would produce broad results that do not identify the specific enzyme responsible for this reaction.

Which of the Following Procedures Would Identify the Enzyme That